Molecular Detection of Class 1 Integrons in <i>Burkholderia pseudomallei</i>
نویسندگان
چکیده
منابع مشابه
Detection of gene cassettes in Tn402-like class 1 integrons.
Amplification of the gene cassettes in class 1 integrons by PCR using primers in the 5 conserved segment (5 -CS) and the 3 -CS (7, 8) has been used in hundreds of studies to identify integron-associated resistance genes (Fig. 1A). Equivalent PCR primers that detect cassettes in class 2 integrons, i.e., Tn7 family transposons (18), are also widely used. The approach used to detect integrons in a...
متن کاملMolecular characterization of class 1 integrons in clinical strains of Salmonella typhimurium isolated in Slovakia.
The presence of class 1 integrons was investigated in 156 epidemiologically unrelated Salmonella Typhimurium isolates. Of these 156 isolates, 70 were of definitive phage type DT104 and 86 were strains of various phage type, RDNC and untypable, designated here as non-DT104 strains. Integrons were found in 47 of DT104 isolates (67.1%), while in all strains with characteristic pentaresistance (R-t...
متن کاملMolecular diversity of class 1 integrons in human isolates of Aeromonas spp. from southern Taiwan.
This work studies antimicrobial resistance and class 1 integrons of Aeromonas spp. in human isolates from southern Taiwan. PCR amplification and DNA sequence analyses were performed to characterize the gene cassette regions of the class 1 integron in 204 isolates of Aeromonas hydrophila, 36 isolates of A. sobria, 23 isolates of A. veronii, and 4 isolates of A. caviae. By using Southern hybridiz...
متن کاملdetection of class 1, 2, and 3 integrons among klebsiella pneumoniae isolated from children in tehran hospitals
background: ctx-m-type β-lactamases are increasingly becoming the predominant esbls globally in recent years. integrons are genetic elements which can integrate gene cassettes, usually antibiotic resistance genes. objectives: the aims were to determine antibiotic susceptibility and to detect genes encoding ctx-m-1 group enzymes and class 1, 2, and 3 integrons among the klebsiella pneumoniae iso...
متن کاملDetection and characterization of class 1 integrons among carbapenem-resistant isolates of Acinetobacter spp. in Malaysia.
BACKGROUND AND PURPOSE To detect and characterize class 1 integrons among carbapenem-resistant strains of Acinetobacter spp. at University Malaya Medical Centre (UMMC), Kuala Lumpur, Malaysia. METHODS Thirty nine carbapenem-resistant Acinetobacter strains were obtained from UMMC from August 2003 to March 2004 and analyzed for the presence of bla(IMP) genes and class 1 integrons. RESULTS Cla...
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ژورنال
عنوان ژورنال: Problems of Particularly Dangerous Infections
سال: 2011
ISSN: 0370-1069
DOI: 10.21055/0370-1069-2011-2(108)-46-48